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Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used plant transformation vectors are termed binary vectors because of their ability to replicate in both ''E. coli'', a common lab bacterium, and ''Agrobacterium tumefaciens'', a bacterium used to insert the recombinant (customized) DNA into plants. Plant Transformation vectors contain three key elements; * Plasmids Selection (creating a custom circular strand of DNA) * Plasmids Replication (so that it can be easily worked with) * Transfer DNA (T-DNA) region (inserting the DNA into the agrobacteria) ==Steps in plant transformation== Propagate binary vector in ''E. coli'' Isolate binary vector from ''E.coli'' and engineer (introduce a foreign gene) Re-introduce engineered binary vector into ''E. coli'' to amplify Isolate engineered binary vector and introduce into ''Agrobacteria'' containing a modified (relatively small) Ti plasmid Infect plant tissue with engineered ''Agrobacteria'' (T-DNA containing the foreign gene gets inserted into a plant cell genome) In each cell T-DNA gets integrated at a different site in the genome Note: There are many variations to these steps. A custom DNA plasmid sequence can be created and replicated in more than one way. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Plant transformation vector」の詳細全文を読む スポンサード リンク
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